Coral reef microbiome: PacBio SMRT system

August 4, 2017 at 12:33 pm
MiCWA - Microbiome Consortium of Western Australia

High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system

W. Pootakham et al. Currently, very little is known about the composition and structure of bacterial communities across P. lutea reefs. The purpose of this study is twofold: to demonstrate the advantages of using PacBio circular consensus sequencing technology in microbial community studies and to investigate the diversity and structure of P. lutea-associated microbiome in the Indo-Pacific. This is the first metagenomic study of marine environmental samples that utilises the PacBio sequencing system to capture full-length 16S rRNA sequences. Despite the geographical and environmental impacts on the coral-host interactions, we identified a conserved community of bacteria that were present consistently across diverse reef habitats. Finally, we demonstrated the superior performance of full-length 16S rRNA sequences in resolving taxonomic uncertainty of coral associates at the species level

Scientific Reports 7, Article number: 2774


Telethon KIds Institute
Australian Phenomics Facility
Lottery West State Biomedical Facility Genomics
Centre for Comparative Genomics
Australian Genome Research Facility
WA Human Microbiome Collaboration Centre
West Coast Microbiome Network